Acid Phosphatase from Human seminal plasma. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
免疫原
Acid Phosphatase isolated and purified from Human seminal plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Restore by adding 1.0 mL of sterile distilled water
浓度
10.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins.
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
抗原
ACPP
(Acid Phosphatase, Prostate (ACPP))
别名
ACPP / Prostatic Acid Phosphatase
背景
Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase