LDH5 抗体 (Biotin)
LDH5
适用: 兔
WB, IF, EIA, DB, ID, IP, RIA
宿主: 绵羊
Polyclonal
Biotin
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- 抗原 See all LDH5 products
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
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适用
- 兔
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This LDH5 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- 产品特性
- Molar Ratio: Biotin/IgG ~ 6.7
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- 免疫原
- L-Lactic Dehydrogenase LDH5(M4) isoenzyme, isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore by adding 1.0 mL sterile distilled water
- 浓度
- 10 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- 抗原
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
- Abstract
- LDH5 产品
- 背景
- Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4
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