Lactate Dehydrogenase (2H2M) (LDH-3) 抗体
LDH-3
适用: 人
WB, IF, EIA, ID, IP, RIA, DB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Lactate Dehydrogenase (2H2M) (LDH-3)
- 适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- 免疫原
- L-Lactic dehydrogenase, LDH-3(H2M2) isoenzyme, is isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 1.0 mL sterile distilled water.
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- 抗原
- Lactate Dehydrogenase (2H2M) (LDH-3)
- 别名
- LDH3 H2M2 Isozyme
- 背景
- Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-3 Isozyme H2M2
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