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LDHB 抗体 (Biotin)

The Biotin-conjugated 兔 多克隆 anti-LDHB antibody (ABIN568275) specifically detects LDHB in WB, IP, IF, EIA, RIA, DB 和 ID. The antibody is reactive with Cow samples.
产品编号 ABIN568275
发货至: 中国
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Quick Overview for LDHB 抗体 (Biotin) (ABIN568275)

抗原

See all LDHB 抗体
LDHB (Lactate Dehydrogenase B (LDHB))

适用

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Cow

宿主

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克隆类型

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多克隆

标记

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This LDHB antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)
  • 特异性

    L-Lactic Dehydrogenase from Bovine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    产品特性

    Molar Ratio: Biotin/IgG ~5.6

    纯化方法

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    免疫原

    L-Lactic Dehydrogenase is isolated and purified from Bovine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    10 mg/mL

    缓冲液

    PBS, pH 7.2 without preservatives and foreign proteins

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • 抗原

    LDHB (Lactate Dehydrogenase B (LDHB))

    别名

    LDHB

    背景

    Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: EC=1.1.1.27, L-lactate dehydrogenase B chain, LDH heart subunit, LDH-B, LDH-H, LDHB, Renal carcinoma antigen NY-REN-46

    基因ID

    281275

    NCBI登录号

    NP_776525

    UniProt

    Q5E9B1

    途径

    Warburg Effect
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