CELA1 抗体
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Quick Overview for CELA1 抗体 (ABIN568185)
抗原
See all CELA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes Elastase from Porcine panceas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
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纯化方法
- Solid Phase Affinity Chromatography
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免疫原
- Elastase isolated and purified from Porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 40-258) antibody
CELA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 159-257) antibodyCELA1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 44-259) antibodyCELA1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 1-258) antibodyCELA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 80-129) antibodyCELA1 适用: 人, 小鼠, Pig, 大鼠, Cow, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (N-Term) antibodyCELA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人 WB, EIA, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL of sterile distilled water
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浓度
- 2.0 mg/mL
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缓冲液
- PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))
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别名
- Elastase-1
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背景
- Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Synonyms: CELA1, Chymotrypsin-like elastase family member 1, ELA1
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基因ID
- 396766
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NCBI登录号
- NP_998988
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UniProt
- P00772
抗原
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