L-Asparaginase 1 抗体
适用: E. coli
WB, IP, EIA, DB, ID, IF, RIA
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- L-Asparaginase 1
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)
- 特异性
- The antibody recognizes L-Asparaginase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- 免疫原
- L-Asparaginase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 1.0 mL of sterile distilled water
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- 抗原
- L-Asparaginase 1
- 背景
- Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L Asparaginase isoenzymes: L Asparaginase I, a low affinity enzyme located in the cytoplasm, and L Asparaginase II, a high affinity secreted enzyme.Synonyms: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA
- 基因ID
- 946278
- NCBI登录号
- AP_002386
- UniProt
- P0A962
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