Alcohol Oxidase 抗体
适用: Candida sp.
IF, WB, EIA, ID, IP, RIA, DB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Alcohol Oxidase
-
适用
- Candida sp.
-
宿主
- 兔
-
克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- 免疫原
- Alcohol dehydrogenase isolated and purified from Candida boidinii. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 1.0 mL of sterile distilled water, Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C. Reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
-
- 抗原
- Alcohol Oxidase
- 背景
- Alcohol Oxidase (AOX) is a homooctameric flavoprotein consisting of eight identical subunits of ~74 kD, each containing a flavin adenine dinucleotide molecule (FAD) as a prosthetic group (van der Klei et al., 1991). The protein catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of certain yeasts including Pichia pastoris, Hansenula polymorpha, and Candida boidinii (van der Klei et al., 1991). AOX is normally localized in the matrix of single membrane-bound organelles called peroxisomes. During methanol growth, the peroxisomes also contain large amounts of dihydroxyacetone synthase, the first enzyme in the methanol assimilatory pathway, and catalase (CAT), which converts the hydrogen peroxide generated by oxidases such as AOX into water and oxygen (Veenhuis and Harder, 1991). As a result, peroxisomes, which are small and few in number in glucose-grown cells, are massively induced in methanol-grown cells (Veenhuis and Harder, 1991).Synonyms: AOD1, AOX, MOX, Methanol Oxidase
- 基因ID
- 5477
- UniProt
- Q00922
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