The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
免疫原
Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Restore by adding 1.0 mL of sterile distilled water.
浓度
10.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins.
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
抗原
ACSS2
(Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))
别名
ACSS2
背景
Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: ACAS2, ACSA, Acetate-CoA ligase, Acetyl-CoA synthetase, Acetyl-coenzyme A synthetase cytoplasmic, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme