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Glucuronidase beta 抗体 (Biotin)

This 兔 多克隆 antibody specifically detects Glucuronidase beta in WB, IP, IF, ID, EIA, RIA 和 DB. It exhibits reactivity toward E. coli.
产品编号 ABIN568042
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Glucuronidase beta 抗体 (Biotin) (ABIN568042)

抗原

See all Glucuronidase beta (GUSB) 抗体
Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

适用

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E. coli

宿主

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克隆类型

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多克隆

标记

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This Glucuronidase beta antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
  • 特异性

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    产品特性

    Molar Ratio: Biotin/IgG~ 6.2

    纯化方法

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

    免疫原

    Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Restore by adding 1.0 mL of sterile distilled water.

    浓度

    10 mg/mL

    缓冲液

    PBS, pH 7.2 without preservatives and foreign proteins.

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • 抗原

    Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

    别名

    beta-Glucuronidase

    背景

    Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB

    基因ID

    946149

    NCBI登录号

    AP_002238

    UniProt

    P05804

    途径

    Glycosaminoglycan Metabolic Process
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