Glucuronidase beta 抗体
Quick Overview for Glucuronidase beta 抗体 (ABIN568041)
抗原
See all Glucuronidase beta (GUSB) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
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纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
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免疫原
- Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore by adding 1.0 mL of sterile distilled water.
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浓度
- 10.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
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别名
- beta-Glucuronidase
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背景
- Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB
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基因ID
- 946149
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NCBI登录号
- AP_002238
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UniProt
- P05804
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途径
- Glycosaminoglycan Metabolic Process
抗原
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