COL6 抗体
Quick Overview for COL6 抗体 (ABIN5680257)
抗原
See all COL6 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human collagen type VI 100 % binding in RIA at 1:10.000 dilution, cross-reactivity with human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % Human, cross-reactive with bovine
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交叉反应
- 小鸡, 牛, 小鼠, 猪, 大鼠
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交叉反应 （详细）
- Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)
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产品特性
- Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100 % , human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % (RIA at 1:10.000 dilution).
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纯化方法
- Affinity purified
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免疫原
- Purified collagen type VI from human placenta
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亚型
- IgG
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anti-Collagen, Type VI (COL6) antibody
COL6 适用: 人, Cow WB, ELISA, IHC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA, IHC, IHC (p), IHC (fro), ICC, DB 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibody (Biotin)COL6 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type VI (COL6) (AA 1-336) antibodyCOL6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, Cow WB, ELISA, IHC, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, 哺乳动物 WB, ELISA, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human placenta,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- 1 mg/mL
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缓冲液
- phosphate buffered solution, no BSA and preservative added!
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储存液
- Without preservative
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储存条件
- -20 °C
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- COL6 (Collagen, Type VI (COL6))
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别名
- Collagen Type VI
抗原
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