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Fibronectin 抗体

This 兔 多克隆 anti-Fibronectin antibody specifically detects Fibronectin in IF, ELISA, IHC (p) 和 RIA. The antibody is reactive with 小鸡 samples.
产品编号 ABIN5680162
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Fibronectin 抗体 (ABIN5680162)

抗原

See all Fibronectin 抗体
Fibronectin

适用

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小鸡

宿主

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克隆类型

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多克隆

标记

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This Fibronectin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
  • 特异性

    Chicken fibronectin 100 % , chicken collagen type I, III and V <0.1 % (RIA at 1:10000 dilution)

    交叉反应 (详细)

    Chicken

    产品特性

    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Chicken fibronectin 100 % , chicken collagen type I, III and V <0.1 % (RIA at 1:10000 dilution).

    纯化方法

    Affinity purified

    免疫原

    Purified fibronectin from chicken plasma

    亚型

    IgG
  • 应用备注

    After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken skin,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

    浓度

    1 mg/mL

    缓冲液

    Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

    储存液

    Without preservative

    储存条件

    -20 °C
  • 抗原

    Fibronectin
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