Fibronectin 抗体
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- 抗原 See all Fibronectin 抗体
- Fibronectin
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Fibronectin antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
- 特异性
- Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution)
- 交叉反应 (详细)
- Cattle, cross-reaction with dog
- 产品特性
- This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution).
- 纯化方法
- Affinity purified
- 免疫原
- Purified fibronectin from bovine plasma
- 亚型
- IgG
- Top Product
- Discover our top product Fibronectin Primary Antibody
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- 应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:80, IHC(P) 1:500 (Envision), ELISA 1:200 (OD>500)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.
- 浓度
- 1 mg/mL
- 缓冲液
- Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!
- 储存液
- Without preservative
- 储存条件
- -20 °C
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- 抗原
- Fibronectin
- Abstract
- Fibronectin 产品
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