Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution)
交叉反应 (详细)
Cattle, cross-reaction with dog
产品特性
This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution).
After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:80, IHC(P) 1:500 (Envision), ELISA 1:200 (OD>500)
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.
浓度
1 mg/mL
缓冲液
Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!