FITC 抗体
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北京 101111
Quick Overview for FITC 抗体 (ABIN567764)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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免疫原
- Highly purified Fluorescein Isothiocyanate isomer 1
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL sterile distilled water
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2 without foreign proteins.
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储存液
- Without preservative
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注意事项
- Avoid repeated thawing and freezing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
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- FITC (Fluorescein Isothiocyanate (FITC))
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别名
- FITC
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物质类
- Chemical
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背景
- Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate
抗原
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