Recombinant ITGA5 (Volociximab Biosimilar) 抗体
Our Local Distributor
北京 101111
Quick Overview for Recombinant ITGA5 (Volociximab Biosimilar) 抗体 (ABIN5668271)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)], Rabbit IgG, kappa
-
特异性
-
This antibody is specific for human α5β1 integrin. Cross-reactivity studies revealed no binding to other human integrins such as α3β1, αvβ3, or αvβ5. It does, however, bind to α5β1 in the vasculature of cynomolgus monkeys, but not in mice. α5β1 is part of the integrin superfamily-glycoprotein transmembrane receptors that interact with ligands such as fibronectin, vitronectin, laminins, and collagens. This receptor plays a key role in processes including inflammation, cell proliferation, angiogenesis, and tumor metastasis.
Application Notes original Clone: This antibody binds human α5β1 integrin with high affinity (Kd = 0.367 nM, EC₅₀ = 0.2 nM, IC₅₀ = 2.3 nM) and, in chimeric mouse-human IgG4 form, demonstrated potent anti angiogenic and anti tumor effects in preclinical models (laser induced CNV in cynomolgus monkeys, VX2 carcinoma in rabbits) before progressing into Phase II trials in platinum resistant ovarian cancer (Ramakrishnan et al., 2006, PMID: 17024968, Bhaskar et al., 2007, PMID: 17786386). Exclusive binding to α5β1 (no cross reactivity with α3β1, αvβ3, αvβ5) was confirmed by ELISA. Immunohistochemistry (IHC-F) demonstrated binding to cynomolgus vasculature but not to mouse tissue. Studies with recombinant integrin subunits revealed that binding depends specifically on the human α5 chain, while the β1 chain can be of either human or mouse origin, indicating strict specificity for the α5β1 heterodimer (Bukowski et al., 2009, DOI: 10.1007/978-1-59745-332-5). In clinical testing, weekly 15 mg/kg IV dosing achieved trough serum levels >150 μg/mL, with 2-3x accumulation by Day 50, detectable antibody in ascites, reduction in circulating endothelial progenitor cells, and a favorable safety profile. The use of this antibody in IHC-P was demostrated (Bell McGuinn et al., 2011, PMID: 21276608). Clone F200, a Fab derivative of M200, is available upon request.
-
产品特性
- NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
-
纯化方法
- Protein A affinity purified
-
纯度
- > 98 % as determined by SDS-PAGE
-
内毒素水平
- Endotoxin is < 1.0 EU/mg as determined by the LAL method
-
免疫原
- Heterodimeric alpha5beta1Fc fusion protein
-
亚型
- IgG, kappa
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
OriginalSpeciesName: Human
OriginalFormat: IgG4
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS with 0.02 % Proclin 300.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
- ITGA5 (Volociximab Biosimilar)
-
物质类
- Biosimilar
-
背景
- Fibronectin receptor, CD49 antigen-like family member E, Integrin alpha-F, VLA-5, CD49e.
-
UniProt
- P08648
抗原
-
