Recombinant IL2RA (Basiliximab Biosimilar) 抗体
Quick Overview for Recombinant IL2RA (Basiliximab Biosimilar) 抗体 (ABIN5668050)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-IL-2R alpha (CD25) [Basiliximab], Human IgG1, kappa
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特异性
- Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
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交叉反应
- Cynomolgus, 猕猴
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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纯度
- > 98 % as determined by SDS-PAGE
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内毒素水平
- Endotoxin is < 1.0 EU/mg as determined by the LAL method
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免疫原
- Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
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亚型
- IgG1 kappa
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anti-IL2RA (Basiliximab Biosimilar) antibody
适用: 人, Cynomolgus, 猕猴 FACS, BR, IF, IHC, WB 宿主: 兔 Chimeric unconjugated Recombinant Antibody
anti-IL2RA (Basiliximab Biosimilar) antibodyResearch Grade 适用: 人 FACS, ELISA 宿主: 小鼠 Chimeric unconjugated Recombinant Antibody
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应用备注
- Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1κ constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
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说明
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NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- IL2RA (Basiliximab Biosimilar)
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背景
- Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD25.
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UniProt
- P01589
抗原
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