KDEL 抗体 (N-Term)
Quick Overview for KDEL 抗体 (N-Term) (ABIN5650489)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Detects KDEL tagged proteins.
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纯化方法
- Protein G Purified
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免疫原
- KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
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亚型
- IgG1
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anti-KDEL (Lys Asp Glu Leu) antibody
Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-KDEL (Lys Asp Glu Leu) antibody (HRP)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal HRP
anti-KDEL (Lys Asp Glu Leu) antibody (FITC)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-KDEL (Lys Asp Glu Leu) antibody (Biotin)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-KDEL (Lys Asp Glu Leu) antibody (PE)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal PE
anti-KDEL (Lys Asp Glu Leu) antibody (APC)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal APC
anti-KDEL (Lys Asp Glu Leu) antibody (PerCP)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal PerCP
anti-KDEL (Lys Asp Glu Leu) antibody (Atto 488)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-KDEL (Lys Asp Glu Leu) antibody (Atto 594)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-KDEL (Lys Asp Glu Leu) antibody (Atto 390)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
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应用备注
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- WB (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5650489 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
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储存条件
- -20 °C
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- KDEL (Lys Asp Glu Leu)
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别名
- KDEL
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背景
- The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
抗原
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