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KDEL 抗体 (N-Term)

The 小鼠 单克隆 anti-KDEL antibody (Clone 2C1) (ABIN5650489) specifically detects KDEL in WB 和 IHC. The antibody is reactive with 小鼠, 大鼠 和 人 samples.
产品编号 ABIN5650489
发货至: 中国
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Quick Overview for KDEL 抗体 (N-Term) (ABIN5650489)

抗原

KDEL (Lys Asp Glu Leu)

适用

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小鼠, 大鼠, 人

宿主

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小鼠

克隆类型

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单克隆

标记

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This KDEL antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

2C1
  • 抗原表位

    • 9
    • 1
    N-Term

    特异性

    Detects KDEL tagged proteins.

    纯化方法

    Protein G Purified

    免疫原

    KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

    亚型

    IgG1
  • 应用备注

    • WB (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.

    说明

    A 1:1000 dilution of ABIN5650489 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

    储存条件

    -20 °C
  • 抗原

    KDEL (Lys Asp Glu Leu)

    别名

    KDEL

    背景

    The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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