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ADO 抗体

ADO 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5647727
发货至: 中国
  • 抗原 See all ADO 抗体
    ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
    适用
    • 34
    • 20
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 34
    克隆类型
    • 34
    多克隆
    标记
    • 12
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADO antibody is un-conjugated
    应用范围
    • 19
    • 18
    • 15
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    纯化方法
    Antigen affinity purified
    免疫原
    Amino acids E49-E261 from the human protein were used as the immunogen for the ADO antibody.
    亚型
    IgG
  • 应用备注
    Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells
    限制
    仅限研究用
  • 缓冲液
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    储存条件
    -20 °C
    储存方法
    After reconstitution, the ADO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • 抗原
    ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
    别名
    ADO / 2-Aminoethanethiol dioxygenase (ADO 产品)
    背景
    Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
    UniProt
    Q96SZ5
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