Mast Cell Tryptase 抗体
This anti-Mast Cell Tryptase antibody is a 兔 多克隆 antibody detecting Mast Cell Tryptase in IHC (p), WB 和 ELISA. Suitable for 人.
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Quick Overview for Mast Cell Tryptase 抗体 (ABIN5647716)
抗原
See all Mast Cell Tryptase 抗体
Mast Cell Tryptase
适用
人
宿主
兔
克隆类型
多克隆
标记
This Mast Cell Tryptase antibody is un-conjugated
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
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纯化方法
- Antigen affinity purified
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免疫原
- Amino acids H65-P275 from the human protein were used as the immunogen for the Mast Cell Tryptase antibody.
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亚型
- IgG
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anti-Mast Cell Tryptase antibody
适用: 人, 大鼠, Cat, 犬, 猴 IHC (p), WB, ELISA, IF, IHC (fro) 宿主: 小鼠 Monoclonal AA1 unconjugated
anti-Mast Cell Tryptase antibody适用: 人 IHC, IHC (p) 宿主: 小鼠 Monoclonal 10D11 unconjugated
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应用备注
- Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA: 0.1-0.5 μg/mL (human protein tested), request BSA-free format for coating
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- Prior to reconstitution, store at 4°C. After reconstitution, the Mast Cell Tryptase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Mast Cell Tryptase
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背景
- Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
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UniProt
- Q15661
抗原
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