Recombinant VWF 抗体 (AA 1815-1939)
Quick Overview for Recombinant VWF 抗体 (AA 1815-1939) (ABIN5646932)
抗原
See all VWF 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1815-1939
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纯化方法
- Purified
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纯度
- Protein A affinity chromatography
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免疫原
- Amino acids 1815-1939 from the human protein were used as the immunogen for this recombinant vWF antibody.
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亚型
- IgG kappa
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应用备注
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The optimal dilution of the recombinant vWF antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunoprecipitation: 1-2 μg/500 μg lysate,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant vWF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- VWF (Von Willebrand Factor (VWF))
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别名
- von Willebrand Factor / vWF
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背景
- Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
抗原
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