Prohibitin 1 / PHB1 (AA 167-261) 抗体
The 小鼠 单克隆 anti-Prohibitin 1 / PHB1 antibody (Clone PHB-1882) (ABIN5646917) specifically detects Prohibitin 1 / PHB1 in IHC (p) 和 WB.
The antibody is reactive with 人 samples.
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产品编号 ABIN5646917
发货至:
中国
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Our Local Distributor
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Quick Overview for Prohibitin 1 / PHB1 (AA 167-261) 抗体 (ABIN5646917)
抗原
Prohibitin 1 / PHB1
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
质量等级
Carrier-free
克隆位点
PHB-1882
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抗原表位
- AA 167-261
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原理
- PHB Antibody / Prohibitin (azide and preservative free)
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产品特性
- This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.
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亚型
- IgG1, kappa
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anti-Prohibitin 1 / PHB1 (AA 167-261) antibody
适用: 人 IHC (p), WB 宿主: 小鼠 Monoclonal PHB-1882 unconjugated
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应用备注
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Optimal dilution of the PHB antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Prohibitin 1 / PHB1
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背景
- This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
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UniProt
- P35232
抗原
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