EpCAM 抗体 (Extracellular Domain)
Quick Overview for EpCAM 抗体 (Extracellular Domain) (ABIN5646871)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 202-209, Extracellular Domain
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody. The epitope has been mapped to the AA 202-209 region.
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亚型
- IgG1
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应用备注
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Titering of the anti-EpCAM antibody may be required for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Western Blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- EpCAM
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背景
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
抗原
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