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K-RAS 抗体 (AA 1-188)

Name: K-RAS 抗体 (AA 1-188)
SKU: ABIN561621
Availability: OutOfStock

KRAS 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B10-2F2 unconjugated

Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2. (K-RAS 抗体 (AA 1-188))

Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody. (K-RAS 抗体 (AA 1-188))

ELISA

Western Blot detection against Immunogen (46. (K-RAS 抗体 (AA 1-188))

Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. (K-RAS 抗体 (AA 1-188))

KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa . (K-RAS 抗体 (AA 1-188))

5 images

(5 references)

产品编号 ABIN561621

发货至: 中国

Datasheet as PDF

抗原 See all K-RAS (KRAS) 抗体

K-RAS (KRAS) (GTPase Kras (KRAS))
抗原表位
16

11

10

8

6

2

2

2

1

1

1

1

1

1

1
AA 1-188
适用
55

39

32

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1
人
宿主
58

10
小鼠
Compatible Secondaries
克隆类型
57

11
单克隆
标记
30

7

4

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This K-RAS antibody is un-conjugated
应用范围
62

29

25

15

13

13

9

6

6

5

3

1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

原理

Mouse monoclonal antibody raised against a full length recombinant KRAS.

序列

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKCVIM

交叉反应

人

产品特性

Antibody Reactive Against Recombinant Protein.

免疫原

KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

克隆位点

3B10-2F2

亚型

IgG1
anti-GTPase Kras (KRAS) (AA 16-125) antibody
KRAS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F3 unconjugated

anti-GTPase Kras (KRAS) (AA 146-174) antibody
KRAS 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 25-130) antibody
KRAS 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 2-168) antibody
KRAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, 兔, 马, 豚鼠, Cow, Drosophila melanogaster, Pig, Bat, 小鸡, 非洲爪蟾, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (pSer89) antibody
KRAS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, 兔, 马, 豚鼠, Cow, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 1-189) antibody
KRAS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 1-188) antibody
KRAS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E4-1B10 unconjugated

anti-GTPase Kras (KRAS) (C-Term) antibody
KRAS 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Madureira, Bharadwaj, Bydoun, Garant, OConnell, Lee, Waisman: "Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10." in: Oncotarget, Vol. 7, Issue 30, pp. 47720-47737, (2016) (PubMed).

King, Bray, Galbraith, Christie, Fleming: "Evidence for aldosterone-dependent growth of renal cell carcinoma." in: International journal of experimental pathology, Vol. 95, Issue 4, pp. 244-50, (2014) (PubMed).

Ambrosini, Khanin, Carvajal, Schwartz: "Overexpression of DDX43 mediates MEK inhibitor resistance through RAS Upregulation in uveal melanoma cells." in: Molecular cancer therapeutics, Vol. 13, Issue 8, pp. 2073-80, (2014) (PubMed).

Valtorta, Misale, Sartore-Bianchi, Nagtegaal, Paraf, Lauricella, Dimartino, Hobor, Jacobs, Ercolani, Lamba, Scala, Veronese, Laurent-Puig, Siena, Tejpar, Mottolese, Punt, Gambacorta, Bardelli et al.: "KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy. ..." in: International journal of cancer. Journal international du cancer, Vol. 133, Issue 5, pp. 1259-65, (2013) (PubMed).

Fuentes-Calvo, Blázquez-Medela, Santos, López-Novoa, Martínez-Salgado: "Analysis of k-ras nuclear expression in fibroblasts and mesangial cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8703, (2010) (PubMed).
抗原

K-RAS (KRAS) (GTPase Kras (KRAS))

别名

KRAS (KRAS 产品)

别名

wu:fa04e08 antibody, wu:fc14b12 antibody, wu:fc23g10 antibody, wu:fj89d12 antibody, zgc:85725 antibody, Kras2 antibody, c-Ki-ras antibody, p21 antibody, C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, AI929937 antibody, K-ras antibody, Ki-ras antibody, Kras-2 antibody, p21B antibody, ras antibody, k-ras antibody, kras antibody, kras-a antibody, kras-b antibody, rask2 antibody, K-RAS antibody, p21ras antibody, KRAS proto-oncogene, GTPase antibody, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog antibody, Kirsten rat sarcoma viral oncogene homolog antibody, semicolonial-7 antibody, Ras family protein antibody, GTPase KRas antibody, Kirsten rat sarcoma viral oncogene homolog S homeolog antibody, KRAS antibody, kras antibody, Kras antibody, smco-7 antibody, PGTG_03066 antibody, Tsp_02666 antibody, Tsp_01642 antibody, rask antibody, kras.S antibody

物质类

Viral Protein

背景

Full Gene Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms: C-K-RAS,K-RAS2A,K-RAS2B,K-RAS4A,K-RAS4B,KI-RAS,KRAS1,KRAS2,NS3,RASK2

基因ID

3845