Urease 抗体 (HRP)
Quick Overview for Urease 抗体 (HRP) (ABIN5596990)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4382-0100
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Supplier
- Rockland
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原理
- Urease Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
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产品特性
- Synonyms: rabbit anti-Urease Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
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纯化方法
- Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Urease (URE) antibody
URE 适用: Jack Bean WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:500 - 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Urease (URE)
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别名
- Urease
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背景
- Background: Urease is an enzyme involved in nitrogen metabolism. It catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
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UniProt
- P07374
抗原
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