GLN1 抗体 (HRP)
Quick Overview for GLN1 抗体 (HRP) (ABIN5596985)
抗原
See all GLN1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-103-238-0100
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Supplier
- Rockland
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原理
- Glutamine Synthetase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].
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产品特性
- Synonyms: goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
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纯化方法
- Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glutamine Synthetase [native from brevibacterium species]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glutamine Synthetase (GLN1) (AA 1-373) antibody
GLN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 285-373) antibodyGLN1 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 50-250) antibodyGLN1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-750M unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) antibodyGLN1 适用: 小鼠, 大鼠 WB, IHC 宿主: 豚鼠 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glutamine Synthetase has been assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GLN1 (Glutamine Synthetase (GLN1))
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别名
- Glutamine Synthetase
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背景
- Background: Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes.
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基因ID
- 3345165
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UniProt
- Q79VE3
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途径
- Positive Regulation of Peptide Hormone Secretion
抗原
-
