Uricase (ABC3735) 抗体 (HRP)
Quick Overview for Uricase (ABC3735) 抗体 (HRP) (ABIN5596984)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-103-092-0100
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Supplier
- Rockland
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原理
- Uricase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].
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产品特性
- Synonyms: goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody
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纯化方法
- Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Uricase (ABC3735) antibody (Biotin)
ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal Biotin
anti-Uricase (ABC3735) antibodyABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Uricase (ABC3735)
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别名
- ABC3735
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物质类
- Dinucleotide
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背景
- Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localized in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
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基因ID
- 3202700
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NCBI登录号
- YP_177228
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UniProt
- Q5WBJ3
抗原
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