R-Phycoerythrin 抗体 (Alkaline Phosphatase (AP))
(1 reference)
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Quick Overview for R-Phycoerythrin 抗体 (Alkaline Phosphatase (AP)) (ABIN5596963)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-105-387
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Supplier
- Rockland
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原理
- R-Phycoerythrin Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, and R-Phycoerythrin conjugated IgG.
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产品特性
- Synonyms: goat anti-R-Phycoerythrin Antibody Alkaline Phosphatase Conjugation, Alk phos conjugated goat anti-R-Phycoerythrin Antibody
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Phycoerythrin, R- antibody (Texas Red (TR))
WB, DB, FM 宿主: 山羊 Polyclonal Texas Red (TR)
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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: "A pathophysiological role of PDE3 in allergic airway inflammation." in: JCI insight, Vol. 3, Issue 2, (2018) (PubMed).
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: "A pathophysiological role of PDE3 in allergic airway inflammation." in: JCI insight, Vol. 3, Issue 2, (2018) (PubMed).
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- R-Phycoerythrin (Phycoerythrin, R-)
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别名
- R-Phycoerythrin
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物质类
- Chemical
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背景
- Background: Anti-R-Phycoerythrin Antibody Alkaline Phosphatase Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to Alk Phos.
抗原
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