Pepsin A 抗体 (HRP)
Quick Overview for Pepsin A 抗体 (HRP) (ABIN5596959)
抗原
See all Pepsin A 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1376-0100
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Supplier
- Rockland
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原理
- Pepsin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
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产品特性
- Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
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纯化方法
- Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Pepsin [Porcine Stomach]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Pepsin A antibody
适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A (Internal Region) antibody适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A antibody适用: Pig WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Pepsin A antibody (Biotin)适用: Pig WB, IHC, ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Immunohistochemistry Dilution: 1:125 - 1:625
Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.
Western Blot Dilution: 1:200 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Pepsin A
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别名
- PGA
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背景
- Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.
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基因ID
- 396892
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UniProt
- P00791
抗原
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