PAX6 抗体
Quick Overview for PAX6 抗体 (ABIN5596956)
抗原
See all PAX6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-r22
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Supplier
- Rockland
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原理
- PAX6 Antibody
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交叉反应 (详细)
- Cross-reactivity with PAX6 from other sources has not been determined.
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产品特性
- Synonyms: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody
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纯化方法
- Anti-PAX6 was purified from clarified ascites by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1 kappa
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应用备注
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Application Note: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:75,000 - 1:150,000
IF Microscopy Dilution: 1:100
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PAX6 (Paired Box 6 (PAX6))
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别名
- PAX6
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背景
- Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
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基因ID
- 5080
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NCBI登录号
- NP_000271
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UniProt
- P26367
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途径
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
抗原
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