MDH1 抗体 (HRP)
Quick Overview for MDH1 抗体 (HRP) (ABIN5596953)
抗原
See all MDH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-603-145-0100
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Supplier
- Rockland
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原理
- Malate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].
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产品特性
- Synonyms: sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase
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纯化方法
- Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibody
MDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B11-B7 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (C-Term) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 265-334) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 211-223) antibodyVerified MDH1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 101-193) antibodyMDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB 宿主: 小鼠 Monoclonal CPTC-MDH1-1 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-334) antibodyMDH1 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:250
Application Note: Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:50
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
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别名
- MDH1
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背景
- Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.
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基因ID
- 396894
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UniProt
- P11708
抗原
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