Laminin 抗体
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- 抗原 See all Laminin (LN) 抗体
- Laminin (LN)
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Laminin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 (详细)
- Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product LN Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Laminin antibody is suitable for western blotting, IHC, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 - 1:8,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Laminin (LN)
- 别名
- Laminin (LN 产品)
- 别名
- LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody
- 背景
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Synonyms: LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody
Background: Anti-Laminin detects laminin. Laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. The laminins are a family of glycoproteins that are an integral part of the structural scaffolding in almost every tissue of an organism. They are secreted and incorporated into cell-associated extracellular matrices. Defective laminins can cause muscles to form improperly, leading to a form of muscular dystrophy, lethal skin blistering disease and defects of the kidney filter. Anti-Laminin Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
Gene Name: LAMB1
- 基因ID
- 3912
- UniProt
- P07942
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