Laminin 抗体
Quick Overview for Laminin 抗体 (ABIN5596936)
抗原
See all Laminin (LN) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-116-0.5
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Supplier
- Rockland
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原理
- Laminin Antibody
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交叉反应 （详细）
- Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
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产品特性
- Synonyms: rabbit anti-Laminin antibody, LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody
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纯化方法
- Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Laminin (LN) (AA 901-1000) antibody
LN 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 兔, Avian, 小鸡, 犬, 鸭 IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) (AA 31-270) antibodyLN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D9G5 unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, IF, IHC (p), IHC (fro), ICC, Func 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Laminin (RABBIT) Antibody has been tested by IHC and was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 μg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 - 1:8,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Laminin (LN)
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别名
- Laminin
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背景
- Background: Anti-Laminin detects laminin. Laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. The laminins are a family of glycoproteins that are an integral part of the structural scaffolding in almost every tissue of an organism. They are secreted and incorporated into cell-associated extracellular matrices. Defective laminins can cause muscles to form improperly, leading to a form of muscular dystrophy, lethal skin blistering disease and defects of the kidney filter. Anti-Laminin Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
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基因ID
- 3912
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UniProt
- P07942
抗原
-
