Lactate Dehydrogenase A 抗体 (HRP)
Quick Overview for Lactate Dehydrogenase A 抗体 (HRP) (ABIN5596934)
抗原
See all Lactate Dehydrogenase A (LDHA) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1373-0100
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Supplier
- Rockland
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原理
- Lactate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
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纯化方法
- Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Lactate Dehydrogenase A (LDHA) antibody
LDHA 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) antibodyLDHA 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (Middle Region) antibodyLDHA 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 豚鼠, 犬, 山羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 154-182) antibodyLDHA 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 204-232) antibodyLDHA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 5-323) antibodyLDHA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (C-Term) antibodyLDHA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:6,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Lactate Dehydrogenase A (LDHA)
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别名
- LDHA
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背景
- Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.
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基因ID
- 100009107, 126050
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UniProt
- P13491
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途径
- Warburg Effect
抗原
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