EDC3 抗体 (pSer161)
Quick Overview for EDC3 抗体 (pSer161) (ABIN5596933)
抗原
See all EDC3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer161
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Supplier Product No.
- 600-401-j38
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Supplier
- Rockland
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原理
- EDC3 phospho S161 Antibody
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交叉反应 (详细)
- Anti-EDC3 pS161 is specific for phospho S161 mouse EDC3.
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产品特性
- Synonyms: rabbit anti-EDC3 pS161 Antibody, Enhancer of mRNA-decapping protein 3, YjeF domain-containing protein 1, Edc-3, Edc 3, Yjdc
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纯化方法
- It was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: EDC3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide surrounding the pS161 site of mouse EDC3.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: Anti-EDC3pS161 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 0.5 μg/mL
ELISA Dilution: 1: 10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- EDC3 (Enhancer of mRNA Decapping 3 (EDC3))
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别名
- Edc3
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背景
- Background: EDC3 antibody is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end. It has also been implicated in cell signaling involving the 14-3-3 proteins and related pathways. Anti-EDC3 [pS161] antibody is ideal for investigators interested in Epigenetic and Nuclear Signaling research.
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UniProt
- Q8K2D3
抗原
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