FOLR1 抗体 (HRP)
Quick Overview for FOLR1 抗体 (HRP) (ABIN5596925)
抗原
See all FOLR1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-103-148-0100
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Supplier
- Rockland
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原理
- Folate Binding Protein Antibody Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].
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产品特性
- Synonyms: goat anti-Folate Binding Protein Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP
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纯化方法
- Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Folate Binding Protein [Bovine Milk]
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Application Note: Anti-Folate Binding Protein has been tested in western blot and is suitable to be assayed against 1.0 μg of Folate Binding Protein [Bovine Milk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- FOLR1 (Folate Receptor 1 (Adult) (FOLR1))
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别名
- FOLR1
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背景
- Background: Folate-binding protein (FBP) are proteins that bind folate, typically the folate receptors and reduced folate carrier. In cancer research it can be a marker for ovarian cancer. It can be measured to help in tumor classification to aid treatment. FBP is over-expressed (20-80 fold) in more than 90 % of ovarian and endometrial cancers, as well as 20-50 % of breast, lung, colorectal, and renal cell carcinomas. FBP has very limited tissue distribution and expression in non-malignant tissue, making it a good immunotherapy target.
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基因ID
- 539750
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NCBI登录号
- NP_001193456
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UniProt
- P02702
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途径
- Dicarboxylic Acid Transport
抗原
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