Mammary Tumor Virus (MMTV) 抗体
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北京 101111
Quick Overview for Mammary Tumor Virus (MMTV) 抗体 (ABIN5596923)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-p12
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Supplier
- Rockland
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原理
- MMTV Antibody
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产品特性
- Synonyms: rabbit anti-MMTV antibody, rabbit anti-mouse mammary tumor virus capsid protein antibody, Gag-Pro-Pol, Mouse mammary tumor virus (strain C3H), MMTV capsid, Reverse transcriptase/ribonuclease H, Capsid protein p27
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纯化方法
- Mouse mammary tumor virus capsid antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01 % and cross adsorbed with 6X HIS.
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过滤
- Sterile filtered
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免疫原
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Immunogen: MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus capsid protein tagged with His.
Immunogen Type: Native Protein
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anti-Mammary Tumor Virus (MMTV) antibody
MMTV 适用: Mouse Mammary Tumor Virus (MMTV) ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 26.7 kDa in size corresponding to MMTV capsid protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000-1:2000
ELISA Dilution: 1:10,000-1:20,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 77 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Mammary Tumor Virus (MMTV)
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别名
- MMTV
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物质类
- Viral Protein
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Enzyme, and Viral research. Mouse mammary tumor virus capsid is a polyprotein that is cleaved by aspartyl protease during or after the release of the virion from the plasma membrane. After entering the cell, reverse transcriptase converts the viral dimeric RNA genome into dsDNA in the cytoplasm. Displaying DNA polymerase activity and RNase H activity, this enzyme copies either DNA or RNA templates and cleaves the RNA strand of the RNA-DNA heteroduplex in a partially processive 3' to 5' endonucleasic mode. tRNS binds to the primer at the 5' end of the viral RNA. Reverse transcriptase uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis proceeding through the U5 region and ending after the repeated region. This RNA-DNA heteroduplex is digested by the RNase H and hybridizes with the identical R region at the 3' end of the viral RNA. RNase H then digests the RNA template except for a polypurine tract situated at the 5' end of the genome. RNase H probably can proceed both in a polymerase-dependent and a polymerase-independent mode. Reverse transcriptase also performs DNA-directed plus-strand DNA synthesis using the polypurine tract that has not been removed by RNase H as primers. Polypurine tract and tRNA primers are then removed by RNase H and the 3' and 5' ssDNA primer binding site regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by reverse transcriptase to the primer binding site and polypurine tract ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats at both ends. Anti-MMTV Antibody is ideal for researchers interested in Cancer, Enzyme, and Viral research.
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UniProt
- P11283
抗原
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