Trypsin Inhibitor 抗体 (HRP)
Quick Overview for Trypsin Inhibitor 抗体 (HRP) (ABIN5596917)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4379-0100
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Supplier
- Rockland
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原理
- Trypsin Inhibitor Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor (Soy Bean).
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产品特性
- Synonyms: rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
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纯化方法
- Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Trypsin Inhibitor peroxidase conjugated antibody has been tested by western blotting and ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Trypsin Inhibitor
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背景
- Background: Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research.
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基因ID
- 547831
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UniProt
- P01070
抗原
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