CPB1 抗体 (HRP)
Quick Overview for CPB1 抗体 (HRP) (ABIN5596913)
抗原
See all CPB1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-403-049-0100
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Supplier
- Rockland
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原理
- Carboxypeptidase B Antibody HRP Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].
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产品特性
- Synonyms: rabbit anti-Carboxypeptidase B antibody HRP conjugation, peroxidase conjugated rabbit anti-Carboxypeptidase B Antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody
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纯化方法
- Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Carboxypeptidase B [Porcine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 111-417) antibody
CPB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 109-415) antibodyCPB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 14-417) antibodyCPB1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 1-417) antibodyCPB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 9-37), (N-Term) antibodyCPB1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB31698 unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) antibodyCPB1 适用: 人, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 16-45), (N-Term) antibodyCPB1 适用: 人 WB, IHC (p), IF, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 1-417) antibodyCPB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (C-Term) antibodyCPB1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 120-415) antibodyCPB1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
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别名
- CPB1
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背景
- Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.
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基因ID
- 397341
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NCBI登录号
- NP_999334
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UniProt
- P09955
抗原
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