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CA2 抗体 (HRP)

Rockland 200-4357-0100 CA2 适用: Cow WB, IHC, ELISA, DB 宿主: 兔 Polyclonal HRP
Rockland
产品编号 ABIN5596909
Supplier Product No.: 200-4357-0100
发货至: 中国
  • 抗原 See all CA2 抗体
    CA2 (Carbonic Anhydrase II (CA2))
    适用
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    宿主
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    多克隆
    标记
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    This CA2 antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB)
    Supplier Product No.
    200-4357-0100
    Supplier
    Rockland
    原理
    Carbonic Anhydrase II Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
    产品特性
    Synonyms: rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
    纯化方法
    Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product CA2 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Carbonic anhydrase II Peroxidase Conjugated Antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    CA2 (Carbonic Anhydrase II (CA2))
    别名
    CA2 (CA2 产品)
    背景
    Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
    基因ID
    280740
    NCBI登录号
    NP_848667
    UniProt
    P00921
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