CA2 抗体 (HRP)
Quick Overview for CA2 抗体 (HRP) (ABIN5596909)
抗原
See all CA2 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4357-0100
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Supplier
- Rockland
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原理
- Carbonic Anhydrase II Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
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产品特性
- Synonyms: rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
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纯化方法
- Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibody
CA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibodyCA2 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (C-Term) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 54-83), (N-Term) antibodyCA2 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB18368 unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 201-260) antibodyCA2 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Carbonic anhydrase II Peroxidase Conjugated Antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CA2 (Carbonic Anhydrase II (CA2))
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别名
- CA2
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背景
- Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
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基因ID
- 280740
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NCBI登录号
- NP_848667
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UniProt
- P00921
抗原
-
