Glucose-6-Phosphate Dehydrogenase 抗体 (HRP)
Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (HRP) (ABIN5596888)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1353-0100
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Supplier
- Rockland
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原理
- Glucose-6-Phosphate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate-Dehydrogenase [Yeast].
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产品特性
- Synonyms: goat anti-Glucose-6-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody
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纯化方法
- Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glucose-6-Phosphate-Dehydrogenase [Yeast]
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Application Note: Anti-Glucose-6-Phosphate Dehydrogenase has been tested in ELISA and western blot. This product is assayed against 1.0 μg of Glucose-6-Phosphate-Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- Glucose-6-Phosphate Dehydrogenase
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背景
- Background: Glucose-6-Phosphate Dehydrogenase is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), which maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Cell growth and proliferation are affected by Glucose-6-Phosphate Dehydrogenase. Glucose-6-Phosphate Dehydrogenase inhibitors are under investigation to treat cancers and other conditions. DHEA is a Glucose-6-Phosphate Dehydrogenase inhibitor.
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基因ID
- 6063509
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UniProt
- P11411
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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