Phosphoserine 抗体 (Biotin)
Quick Overview for Phosphoserine 抗体 (Biotin) (ABIN5596874)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-261
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Supplier
- Rockland
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原理
- Phosphoserine Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.
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产品特性
- Synonyms: phospho-Ser antibody, pS antibody, pSER antibody, Phosphoserine [pS] reactive antibody, Phosphoserine [pS] polyclonal antibody, Phosphoserine antibody produced in rabbit
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纯化方法
- Anti-PhosphoSerine (pS) (RABBIT) Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-PhosphoSerine (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Immunogen Type: Other
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亚型
- IgG
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anti-Phosphoserine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine (phosphorylated) antibodyWB 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 594)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphoserine antibody (Atto 390)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-PhosphoSerine Antibody Biotin Conjugated reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. Anti-PhosphoSerine Antibody Biotin Conjugated reacts with free phosphoserine, phospho-serine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry. Specific conditions should be optimized by user.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Phosphoserine
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物质类
- Amino Acid
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背景
- Background: PhosphoSerine Antibody Biotin Conjugated is suitable for use in ELISA and western blotting assays. Phosphoserine is an ester of serine and phosphoric acid. Phosphoserine is a component of many proteins as the result of posttranslational modifications. The phosphorylation of the alcohol functional group in serine to produce phosphoserine is catalyzed by various types of kinases. Phosphoserine antibody will detect proteins phosphorylated on serine residues. Anti-PhosphoSerine antibody is ideal for investigators involved in Cancer, Neuroscience and Cell Signaling Research.
抗原
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