GPD1 抗体 (HRP)
Quick Overview for GPD1 抗体 (HRP) (ABIN5596858)
抗原
See all GPD1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-103-210-0100
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Supplier
- Rockland
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原理
- Glycerol-3-Phosphate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody
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纯化方法
- Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibody
GPD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C10-1C4 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 28-57), (N-Term) antibodyGPD1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21027 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) antibodyGPD1 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 35-65), (N-Term) antibodyGPD1 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 110-159) antibodyGPD1 适用: 人, 小鼠, 犬, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 251-300) antibodyGPD1 适用: 人, 斑马鱼, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (N-Term) antibodyGPD1 适用: 人, 小鼠, 兔, 大鼠, 犬, 豚鼠, 马, Cow, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glycerol-3-Phosphate Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
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别名
- GPD1
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背景
- Background: Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. Through the reduction of dihydroxyacetone phosphate into glycerol 3-phosphate, GPDH allows the prompt dephosphorylation of glycerol 3-phosphate into glycerol. It is also a major contributor of electrons to the electron transport chain in the mitochondria. GPDH is responsible for maintaining the redox potential across the inner mitochondrial membrane in glycolysis. Since glycerol is a main subunit in lipid metabolism, its abundance can easily lead to an increase in triglyceride accumulation at a cellular level. As a result, there is a tendency to form adipose tissue leading to an accumulation of fat that favors obesity. GPDH has also been found to play a role in Brugada syndrome. Mutations in the gene encoding GPD1 have been proven to cause defects in the electron transport chain. This conflict with NAD+/NADH levels in the cell is believed to contribute to defects in cardiac sodium ion channel regulation and can lead to a lethal arrythmia during infancy.
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基因ID
- 100339469, 3043365
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UniProt
- P08507
抗原
-
