COL6 抗体 (HRP)
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- 抗原 See all COL6 抗体
- COL6 (Collagen, Type VI (COL6))
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适用
- 人, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL6 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 600-403-108
- Supplier
- Rockland
- 原理
- Collagen Type VI Antibody Peroxidase Conjugated
- 交叉反应 (详细)
- Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
- 产品特性
- Synonyms: rabbit anti-Collagen Type VI antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
- 纯化方法
- Anti-Collagen VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Collagen Type VI from human and bovine placenta
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL6 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Collagen Type VI (RABBIT) Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 50μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal - 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- COL6 (Collagen, Type VI (COL6))
- 别名
- Collagen Type VI (COL6 产品)
- 背景
- Background: Collagen Type VI Peroxidase Conjugated Antibody is specific for collagen type VI protein. The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
- 基因ID
- 1291
- UniProt
- P12109
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