COL6 抗体
Quick Overview for COL6 抗体 (ABIN5596845)
抗原
See all COL6 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-108-0.5
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Supplier
- Rockland
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原理
- Collagen Type VI Antibody
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交叉反应 （详细）
- Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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产品特性
- Synonyms: rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
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纯化方法
- Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Collagen Type VI from human and bovine placenta
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Collagen, Type VI (COL6) antibody
COL6 适用: 人, Cow WB, ELISA, IHC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA, IHC, IHC (p), IHC (fro), ICC, DB 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibody (Biotin)COL6 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type VI (COL6) (AA 1-336) antibodyCOL6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, Cow WB, ELISA, IHC, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, 哺乳动物 WB, ELISA, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Collagen Type VI has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- COL6 (Collagen, Type VI (COL6))
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别名
- Collagen Type VI
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背景
- Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
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基因ID
- 1291
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UniProt
- P12109
抗原
-
