Collagen, Type I (COL1) 抗体

ABIN5596819 产品详细信息
抗原
  • collagen type III alpha 1 chain
  • COL3A1
适用
牛, 人, 哺乳动物, 小鼠, 大鼠
205
91
88
35
16
11
10
10
10
9
7
7
7
6
4
3
2
2
2
2
2
1
1
宿主

192
67
37
克隆类型
多克隆
标记
非结合性
31
10
7
3
3
3
2
2
2
2
2
2
1
1
1
1
1
应用范围
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
198
127
93
93
85
82
69
38
27
27
21
15
15
8
7
5
5
5
2
1
1
选项
'独立验证'标志
抗原 COL1
Lot Number 41897
Method validated Unfolding Profile
Positive Control ABIN5596819
Notes

Passed. ABIN5596819 showed Ti at 79.6°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

实验流程
  • Dilute ABIN5596819 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validation Images
Unfolding Profile validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Unfolding profile of ABIN5596819. The fluorescence signal is plotted against temperat...
免疫原

Immunogen: Collagen Type I from human and bovine placenta

Immunogen Type: Native Protein

亚型 IgG
交叉反应 (详细) Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
纯化方法 COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
抗原
别名 COLLAGEN Type I
背景

Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL1A1

基因ID 1277
UniProt P02452
应用备注

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formali...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. L...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to d...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fi...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1186/1471-2474-11-10: The testing of multi-differentiation of r...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Rabbit Anti-collagen type I antibody. Tissue: right lobe of t...
Immunostaining (ISt) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1371/journal.pone.0009730: Both serum-free and serum-containing...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1371/journal.pone.0012501: Age-related alterations in collagen ...
Flow Cytometry (FACS) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1016/j.jim.2010.09.025: Expression of collagen by 5 day culture...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1002/hep.25754: ASH1 positively influences the expression of TI...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.4081/ejh.2012.e29: Immunoperoxidase stain for collagen type I (...
Flow Cytometry (FACS) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.4049/jimmunol.1200604: CCR2 mediates the specific accumulation ...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1016/j.tice.2013.03.001: Pulmonary fibroblasts deposit elastin ...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1016/j.yjmcc.2013.07.005: AICAR treatment reduces post-MI adver...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1371/journal.pone.0091141: Collagen Far Western blot of B. frag...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1016/j.jss.2014.03.036: Oridonin suppresses α-SMA and ECM prote...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1002/path.4530: Inhibition of collagen prolyl hydroxylase by PD...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1093/hmg/ddv154: SMT022357 improves the pathology in the diaphr...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1080/15476286.2014.996467: SIM mutant as dominant negative prot...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1016/j.jss.2015.07.042: CYD0682 suppresses endogenous ECM prote...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1371/journal.pone.0147804: Immunofluorescence and SHG detection...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/srep22597: Reduced secretion of type I collagen by Akt inh...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.3892/ijmm.2016.2582: Zoledronic acid (ZA) at the maximum concen...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/srep33836: Collagen type I expression in the IVD tissue. A...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/srep41173: Inhibition of mTORC1 reduces secretion of type ...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/srep42563: Anti-fibrotic effect of apigenin in human HSC l...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1039/C6RA17459K: HJC0123 inhibits hepatic stellate cells activa...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/ncomms15861: At least two grid per tissue biopsies were pr...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.15252/embj.201695553: p21 knockout alleviates liver fibrosis. A...
Flow Cytometry (FACS) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1007/s00395-017-0623-4: The augmented participation from infilt...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1172/jci.insight.92192: Transgenic VEGFA overexpression reduced...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1007/s11010-015-2562-4: CYD0692 suppresses endogenous and TGF-β...
In situ hybridization (ISH) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1038/s41598-018-21194-3: Decreased gene and protein expression ...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1167/iovs.17-23702: LPA increases α-SMA and ECM production in H...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1371/journal.pone.0197777: Recombinant ACLP inhibits adipogenes...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1186/s12931-018-0876-y: Effect of ex vivo treatment with Pirfen...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1177/2041731418810164: Representative histological analysis for...
Immunostaining (ISt) image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) http://www.doi.org/10.1177/2472630318776594: Histological analysis of muscle and tend...
有引用在: Ho, Skiba, Ullmer, Rao: "Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells." in: Investigative ophthalmology & visual science, Vol. 59, Issue 5, pp. 1969-1984, 2019 (PubMed).

Surolia, Li, Wang, Li, Liu, Zhou, Luckhardt, Bae, Liu, Rangarajan, de Andrade, Thannickal, Antony: "3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs." in: JCI insight, Vol. 2, Issue 2, pp. e91377, 2019 (PubMed).

Bürgi, Kunz, Abrami, Deuquet, Piersigilli, Scholl-Bürgi, Lausch, Unger, Superti-Furga, Bonaldo, van der Goot: "CMG2/ANTXR2 regulates extracellular collagen VI which accumulates in hyaline fibromatosis syndrome." in: Nature communications, Vol. 8, pp. 15861, 2018 (PubMed).

Zhang, Stefanovic: "mTORC1 phosphorylates LARP6 to stimulate type I collagen expression." in: Scientific reports, Vol. 7, pp. 41173, 2018 (PubMed).

Hicks, Goossens, Blas-García, Tsuchida, Wooden, Wallace, Nieto, Lade, Redhead, Cederbaum, Dudley, Fuchs, Lee, Hoshida, Friedman: "Transcriptome-based repurposing of apigenin as a potential anti-fibrotic agent targeting hepatic stellate cells." in: Scientific reports, Vol. 7, pp. 42563, 2018 (PubMed).

Trial, Heredia, Taffet, Entman, Cieslik: "Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis." in: Basic research in cardiology, Vol. 112, Issue 4, pp. 34, 2018 (PubMed).

Cummins, Wang, Nunez Lopez, Graham, Tie, Zhou, Radhakrishnan: "Luteolin-Mediated Inhibition of Hepatic Stellate Cell Activation via Suppression of the STAT3 Pathway." in: International journal of molecular sciences, Vol. 19, Issue 6, 2018 (PubMed).

Lehmann, Buhl, Alsafadi, Klee, Hermann, Mutze, Ota, Lindner, Behr, Hilgendorff, Wagner, Königshoff: "Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 175, 2018 (PubMed).

Jager, Lee, Li, Farmer, Fried, Layne: "Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue." in: PLoS ONE, Vol. 13, Issue 5, pp. e0197777, 2018 (PubMed).

Laternser, Keller, Leupin, Rausch, Graf-Hausner, Rimann: "A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues." in: SLAS technology, Vol. 23, Issue 6, pp. 599-613, 2018 (PubMed).

Shukunami, Takimoto, Nishizaki, Yoshimoto, Tanaka, Miura, Watanabe, Sakuma, Yamamoto, Kondoh, Hiraki: "Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 3155, 2018 (PubMed).

Roselló-Díez, Stephen, Joyner: "Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth." in: eLife, Vol. 6, 2018 (PubMed).

He, Bougioukli, Ortega, Arevalo, Lieberman, McMahon: "Sox9 positive periosteal cells in fracture repair of the adult mammalian long bone." in: Bone, Vol. 103, pp. 12-19, 2018 (PubMed).

Zhang, Stefanovic: "Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen." in: Scientific reports, Vol. 6, pp. 22597, 2017 (PubMed).

Komatsu, Ibi, Chosa, Kyakumoto, Kamo, Shibata, Sugiyama, Ishisaki: "Zoledronic acid suppresses transforming growth factor-β-induced fibrogenesis by human gingival fibroblasts." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 139-47, 2017 (PubMed).

Murray, Habiel, Hohmann, Camelo, Shang, Zhou, Coelho, Peng, Gulati, Crestani, Sleeman, Mustelin, Moore, Ryu, Osafo-Addo, Elias, Lee, Hu, Herazo-Maya, Knight, Hogaboam, Herzog: "Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis." in: JCI insight, Vol. 2, Issue 16, 2017 (PubMed).

Yang, Peng, Gopalan, Gao, Chen, Joyner: "Stromal hedgehog signaling maintains smooth muscle and hampers micro-invasive prostate cancer." in: Disease models & mechanisms, Vol. 10, Issue 1, pp. 39-52, 2017 (PubMed).

Yosef, Pilpel, Papismadov, Gal, Ovadya, Vadai, Miller, Porat, Ben-Dor, Krizhanovsky: "p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling." in: The EMBO journal, Vol. 36, Issue 15, pp. 2280-2295, 2017 (PubMed).

Clevenger, Hinman, Ashley Rubin, Smither, Burke, Hawker, Messina, Van Epps, Clegg: "Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells." in: Tissue engineering. Part A, Vol. 22, Issue 7-8, pp. 597-609, 2017 (PubMed).

Nunez Lopez, Bohanon, Wang, Ye, Corsello, Rojas-Khalil, Chen, Chen, Zhou, Radhakrishnan: "STAT3 Inhibition Suppresses Hepatic Stellate Cell Fibrogenesis: HJC0123, a Potential Therapeutic Agent for Liver Fibrosis." in: RSC advances, Vol. 6, Issue 102, pp. 100652-100663, 2016 (PubMed).

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