Acetylated Lysine 抗体
(3 references)
Quick Overview for Acetylated Lysine 抗体 (ABIN5596809)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-939
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Supplier
- Rockland
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原理
- Lysine Acetylated Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.
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产品特性
- Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody
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纯化方法
- Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Immunogen Type: Other
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亚型
- IgG
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anti-Acetylated Lysine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Acetylated Lysine antibody适用: 小鸡, Cow WB, ELISA, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 7F8 unconjugated
anti-Acetylated Lysine antibodyWB 宿主: 小鼠 Monoclonal 15G10 unconjugated
anti-Acetylated Lysine antibody适用: Various Species WB 宿主: 兔 Polyclonal unconjugated
anti-Acetylated Lysine (acetylated) antibodyWB, IP, IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.
Western Blot Dilution: 1:500-1:1,000
Immunoprecipitation Dilution: 10 μg/mg protein sample
ELISA Dilution: 1:1,000-1:2,500
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." in: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).
: "KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123935, (2016) (PubMed).
: "PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1." in: Nucleic acids research, Vol. 40, Issue 14, pp. 6520-33, (2012) (PubMed).
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: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." in: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).
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- Acetylated Lysine
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别名
- Lysine Acetylated
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物质类
- Chemical
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背景
- Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.
抗原
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