Streptavidin 抗体 (Biotin)
Quick Overview for Streptavidin 抗体 (Biotin) (ABIN5596791)
抗原
See all Streptavidin 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-406-095
-
Supplier
- Rockland
-
交叉反应 （详细）
- No reaction was observed against Avidin.
-
纯化方法
- Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.
-
免疫原
-
Immunogen: Streptavidin (Streptomyces avidinii)
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Streptavidin (AA 25-183) antibody
适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, IF 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody适用: Streptomyces avidinii ELISA, WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentation in immunoassays should be performed by the researcher.
ELISA Dilution: 1:2,000-1:20,000
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Streptavidin
-
背景
-
Synonyms: BAC, Streptavidin Biotin, Anti-Streptavidin BAC Antibody
Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin thorught the antbody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interation.
抗原
-
