CPA1 抗体 (HRP)
Quick Overview for CPA1 抗体 (HRP) (ABIN5596781)
抗原
See all CPA1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4352-0100
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Supplier
- Rockland
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原理
- Carboxypeptidase A Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Carboxypeptidase A antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
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纯化方法
- Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 205-419) antibody
CPA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPA1-2714 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPA1-2711 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3F11 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (Internal Region) antibodyCPA1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-601M unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 201-300) antibodyCPA1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 111-419) antibodyCPA1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Carboxypeptidase A Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers.
Western Blot Dilution: 1:300 - 1:2,000
ELISA Dilution: 1:3,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
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别名
- CPA1
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背景
- Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.
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基因ID
- 286762
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NCBI登录号
- NP_777175
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UniProt
- P00730
抗原
-
