Ascorbate Oxidase 抗体 (HRP)
Our Local Distributor
北京 101111
Quick Overview for Ascorbate Oxidase 抗体 (HRP) (ABIN5596757)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-4348-0100
-
Supplier
- Rockland
-
交叉反应 （详细）
- Cross reactivity against Ascorbate Oxidase from other sources is unknown.
-
纯化方法
- Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
-
免疫原
-
Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Ascorbate Oxidase (AO) (AA 31-579) antibody
AO 适用: Pumpkin ELISA, WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:10,000
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
-
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Ascorbate Oxidase (AO)
-
别名
- Ascorbate Oxidase
-
背景
-
Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.
Gene Name: AAO
-
UniProt
- P24792
抗原
-
