Ascorbate Oxidase 抗体 (HRP)
Quick Overview for Ascorbate Oxidase 抗体 (HRP) (ABIN5596757)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4348-0100
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Supplier
- Rockland
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交叉反应 （详细）
- Cross reactivity against Ascorbate Oxidase from other sources is unknown.
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纯化方法
- Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
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免疫原
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Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Ascorbate Oxidase (AO) (AA 31-579) antibody
AO 适用: Pumpkin ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Ascorbate Oxidase (AO)
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别名
- Ascorbate Oxidase
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背景
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Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.
Gene Name: AAO
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UniProt
- P24792
抗原
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