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- 抗原
- LMW HCP (LMW Host Cell Proteins (LMW HCP))
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LMW HCP antibody is un-conjugated
- 应用范围
- ELISA, Western Blotting (WB)
- 原理
- Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli.
- 交叉反应 （详细）
- Reactivity is directed against low molecular weight E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
- 产品特性
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Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, low molecular weight E.Coli HCP proteins antibody, LMW HCP antibody
Background: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
- 纯化方法
- This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
- 免疫原
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Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli.
Immunogen Type: Native Protein
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anti-LMW Host Cell Proteins (LMW HCP) antibody
LMW HCP 适用: E. coli ELISA, WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot Dilution: 1:500 - 1:1000
ELISA Dilution: 1:1000-1:2000
- 说明
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Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and is suitable for western blotting and ELISA.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 72.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store LMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- LMW HCP (LMW Host Cell Proteins (LMW HCP))
- 别名
- LMW E.Coli Host Cell Protein
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